Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.    Staining of thioglycolate-induced peritoneal exudate cells (PECs) with 0.03 µg of Rat IgG2a kappa Isotype Control APC (Product # 17-4321-81) (blue histogram) or 0.03 µg of Anti-Mouse CD115 (c-fms) APC (purple histogram). Total viable cells were used for analysis.   Figure 1 AGEs increased proportions of CD115 + monocytes and their inflammatory subset in the bone marrow. Mice were divided into control ( n = 11) and AGE ( n = 11) groups. The AGE group received AGEs (25 mg/kg) via intraperitoneal injection once a day for 7 consecutive days, while the control group received BSA. On the 8th day, 5 mice in each group were sacrificed and bone marrow mononuclear cells were isolated for surface marker analysis by flow cytometry. The other 6 mice in each group also were sacrificed, and bone marrow CD115 + monocytes were sorted for real-time PCR analysis. (a) Proportions of CD115 + monocytes in the bone marrow. Bar graphs represent the results (mean +- SD) of five independent experiments. (b) Proportions of CD115 + Ly6C high inflammatory monocytes in the bone marrow. Bar graphs represent the results (mean +- SD) of five independent experiments. (c) mRNA expression of IL-1 beta , TNF- alpha , and IL-10 in CD115 + monocytes. Bar graphs represent the results (mean +- SD) of three independent experiments. * P    Figure 5 EPO increases CCL2-deriven egress of Ly6C hi monocytes from BM. ( A ) Serum CCL2 levels in diluent (control) or EPO-injected mice detected by ELISA, graphs represent mean +- SEM, N = 5, **p    Figure 4 Per1 inhibits Ccr2 expression and macrophage migration. ( a ) Flow cytometry analysis of surface CD115 and CD11b was used to determine the relative numbers of monocytes in peripheral blood. Ccr2 expression was measured in the livers ( b ) and peritoneal macrophages ( c ). * P    Figure 2 Characterization of the immune system of Mphi-c-Myc-KO mice under steady-state conditions. All parameters were analysed in 6 mice per genotype. ( A ) Total numbers of bone marrow cells isolated from mice after flushing femurs and tibias with PBS. ( B ) Flow cytometry analysis of bone marrow precursors. MMPs, LT-HSCs, ST-HSCs were analyzed by double immunostaining for Flk2 and Cd90 within the parental Lin - Sca1 + c-Kit + (LSK) population. MEPs, GMPs and CMs were analyzed by double immunostaining for CD16 and CD34 within the parental LSK population. MPs were detected by staining for CD115 + within the parental Lin - population. ( C ) Clonogenic activity of total bone marrow cells. ( D ) Blood hemogram analysis for the indicated cell types. ( E ) Flow cytometry of blood cells doubly stained for Gr-1 and CD115 to identify neutrophils (Gr-1 high CD115 - ), classical monocytes (Gr-1 high CD115 + ) and non-classical monocytes (Gr-1 - CD115 + ). ( F ) Spleen sections were either stained with hematoxilin/eosin or analyzed by confocal microscopy to visualize macrophage infiltration (CD68+ cells) and nuclei (DAPI staining). Representative images are shown, with higher magnification for CD68 immunofluorescence showing macrophage-rich red pulp surrounding a spleenic follicle. The graph shows the quantification of CD68 + macrophages within the spleen using Imaris software (n = 3 tumors per genotype). Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. CSF1R (colony stimulating factor 1 receptor) is a cytokine that controls the production, differentiation, and function of macrophages. CSF1R mediates most if not all of the biological effects of CSF1. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy. 蛋白别名: c-fms; CD115; CSF-1 receptor; CSF-1-R; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms Host server : magellan-srch-1-prod-green:8080/10.253.224.248:8080. git-commit: 2cd8645d2fc6bfe4ccb4abfa14772b0a94f68e98 git-url: http://victoria.invitrogen.com:8333/magellan/core.git git-branch: origin/release/1.27.0-2021.08.32-1.0